Culture of fish food organisms

Unit 5 - Infusorians

5.2.3.Lettuce culture
Place brown, rotting lettuce leaves are placed in a widemouth glass jar. Put in enough to cover the bottom of the jar. Add water that is almost boiling to fill the jar about 3/4 of the way full. Leave this standing, uncovered for 24 hours. Then add 50-100 ml of old aquarium water (from the surface of the aquarium) and cover the jar. After about one week or ten days, the water in the jar should have a heavy growth of infusoria. Once in every ten days a piece of scalded lettuce should be added to keep the culture growing.