Veterinary Clinical Biochemistry and Laboratory Diagnosis

PROCEDURE

Sample

• Serum
• Reagents Provided
  • Enzyme Regent
  • Buffer solution
  • Glucose standard (100 mg%)

Reagent Preparation - [The reagents in the kit vary from manufacturer to manufacturer, so the procedure in  the kit used may be followed.]

• Dilute one vial of enzyme  in one bottle solution.
• Mix gently to dissolve.
• The prepared working enzyme reagent is stable for at least a month at 2-8^ºC.

Procedure (Agappe diagnostics kit)

• Pipette into clean dry test tubes labeled blank (B), Standard (S) and test (T).

S.No	Reagent	B	S	T
1.	Working Enzyme Reagent	3.0 ml	3.0 ml	3.0 ml
2.	Distilled Water	30 μl	-	-
3.	Glucose standard	-	30 μl	-
4.	Serum / Plasma	-	-	30 μl

• Mix well and incubate at 37^ºC for 15 minutes. Then measure the absorbance of Test (T) and Standard (S) against Blank (B) on a Photocolorimeter with a green filter or on a Spectrophotometer at 505 nm.

Calculation

Result

• The amount of glucose present in the given serum = ---------- mg/dL