Diagnosis and differential diagnosis
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Samples
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Spleen, pre scapular or mesenteric lymph nodes, ocular or nasal secretions of acutely infected animals
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Isolation and identification of the virus - Isolation of the virus from leukocyte fraction of whole blood that has been collected into heparin or EDTA (ethylene diamine tetra acetic acid), monolayer of primary calf kidney, B95a marmoset lymphoblastoid or African green monkey kidney (Vero) cells.
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Glycerol should not be used as preservative for Rinderpest virus.
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Serological tests – AGID, VNT (virus neutralization test), Competitive ELISA
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RT-PCR
Differential diagnosis
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Haemorrhagic septicemia
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FMD
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Mucosal disease
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Bovine Viral Diarrhoea
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Last modified: Sunday, 3 June 2012, 6:54 AM