Veterinary Preventive Medicine-II

DIAGNOSIS

Specimens : Liver and spleen.

• A presumptive diagnosis is made on the basis of clinical signs and gross and microscopic lesions observed in the liver.
• Confirmation requires isolation and identification of the virus.
• The virus replicates on the chorioallantoic membrane of chicken embryos and in various cell cultures including vero, CER, BHK 21, mosquito line cells, primary calf, lamb and goat kidney or testis cells or in suckling and weaned mice or hamsters inoculated intracerebrally or intraperitoneally.
• Cytopathic effect may be evident one to five days after inoculation of cell cultures and the virus identified by FAT.
• Serological tests- by CFT, ELISA, FAT,HI, RPHA, IPT.

Differential diagnosis

• Agents or poisoning caused by poisonous plants such as Seneciao sp, Crotalaria sp, Lasiospermum, pasteurellosis, salmonellosis, anthrax, RP, and PPR.