Systematic Veterinary Virology

CULTIVATION

• Tissue culture systems are ideal for the cultivation of PPRV. The virus grows well in lamb kidney cells and African green monkey cells (Vero cells).
• The CPE develop within 5 days and consists of cell rounding and aggregation and syncytia formation in lamb kidney cells. In Vero cells syncytia are rare and if present are very small.
• Syncytia are recognised by a circular arrangement of nuclei giving a ‘clock face’ appearance. Other important CPE include intracytoplasmic and intranuclear inclusions and vacuolation of cells.