Systematic Veterinary Virology

CULTIVATION

• Cell culture: CDV grows well in dog kidney cells, ferret cells and chick fibroblasts. Formation of cytoplasm and acidophilic intracytoplasmic and intranuclear inclusion bodies are characteristic CPEs. 
• Embryonated eggs: The virus also grows in the Chorioallantoic membrane. However, no changes are produced upto 10^th passage. Subsequent passages show increased thickening and whitish areas in the chorioallantoic membrane.
• Laboratory animals: The distemper viruses are cultivated in live dogs or ferrets. Few strains also grown in suckling mouse.