Breeding of Vegetable, Spice and Tuber crops (2+1)

DNA Amplification Fingerprinting (DAF)

 

• DNA Amplification Fingerprinting (DAF) is one of the recent amplification based nucleic acid scanning techniques. DAF utilizes primers of 5-12 nts of length of arbitrary sequence.

• Primers as short as 5 nts in length can produce complex banding patterns that are resolved by polyacrylamide gel electrophoresis and silver staining.

• DAF uses low stringency amplification condition, so that primers can anneal arbitrarily at multiple sites on template DNA strand and initiate DNA synthesis.

• DAF does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins.
  

• DAF can be used to assess genetic diversity, to identify genotype and also for segregation and linkage analysis.