DNA Amplification Fingerprinting (DAF)

DNA Amplification Fingerprinting (DAF)
  • DNA Amplification Fingerprinting (DAF) is one of the recent amplification based nucleic acid scanning techniques. DAF utilizes primers of 5-12 nts of length of arbitrary sequence.
  • Primers as short as 5 nts in length can produce complex banding patterns that are resolved by polyacrylamide gel electrophoresis and silver staining.
  • DAF uses low stringency amplification condition, so that primers can anneal arbitrarily at multiple sites on template DNA strand and initiate DNA synthesis.
  • DAF does not depend on cloning or DNA sequence information and can generate fingerprints from DNA of viral, bacterial, fungal, plant and animal origins.
  • DAF can be used to assess genetic diversity, to identify genotype and also for segregation and linkage analysis.

Last modified: Monday, 2 April 2012, 11:22 PM