Veterinary Andrology and Reproductive Techniques

DILUENT PREPARATION

The diluents preparation involves two steps

• Preparation of buffer
• Preparation of diluents

Points to be considered before preparation of diluent

• The chemicals should be purchased from reliable source.
• The compounds used should be chemically pure and weighing should be accurate.
• The triple glass distilled water or Millipore water should be used for dissolving the salts.
• Completely dissolve one salt before adding another salt.
• The buffer solution should be sterilized by autoclaving before use.