Principles of Biochemistry (2+1)

Preparation of normal solution and standardisation

·    0.1 N sodium carbonate solution: Weight out exactly 1.3250 g of Na₂CO₃ and dissolve in 250 ml distilled water.

·    0.1 N Sulphuric acid: Pour 3 ml of the Conc. H₂SO₄ careful into 10-12 ml of water, cool, mix thoroughly and dilute to 1 L.

·    To standardize, titrate against 0.1N Na₂CO₃ using methyl red as indicator.

·    1 N Sodium hydroxide: Dissolve 4g of NaOH pellets in water cool and dilute to 100ml in a volumetric flask.

·    Dilute to get 0.1N solution.

·    Standardize NaOH by titrating against Std. H₂SO₄ solution using phenolphthalein as indicator.

·    0.1 N Hydrochloric acid: Pour 9 ml of Conc. HCI carefully into 10-12ml of distilled water, cool, mix thoroughly and dilute to 1 L.

·    To standardize, titrate against 0.1N NaOH using Phenolphthalein as indicator.