Principles of Biochemistry (2+1)

Calculation

 Total N % by weight   =       0.14 (V – U) x 100x 100

                                           ---------------------------------                                                    0.5x 5X1000  

1 ml of 0.01N H₂SO₄ = 0.14 mg of N.

U =     Volume in ml of H₂SO₄ solution used to neutralize the

          ammonia liberated from blank solution

V =     Volume in ml of H₂SO₄ solution used to neutralize the ammonia

          liberated from the sample solution

W=    Weight of (in g) material taken for the test.

     Total protein %  x N content X 6.25