Physiology of Finfish and Shellfish (2+1)

Reagents

1. Managanous sulfate (MnSO₄) solution: 480g MnSO₄.4H₂O or 400g MnSO₄.2H₂O or 364 g of MnSO₄ H₂O is dissolved in distilled water and the volume is made upto 1l.
2. Alkaline iodide: 500g of NaOH or 700g of KOH and 135g BaI or 150g KI is dissolved with 1l distilled water.
3. Concentrated H₂SO₄
4. Starch indicator (1%) – This should be prepared at the time of need by adding 1g starch in 1l distilled water.
5. Sodium thiosulphate solution (0.01N) is dissolved in previously boiled distilled water and g of Na₂S₂O₃ the volume is made upto 1l. The solution is stored in a coloured bottle and may be preserved with 2 or 3 pellets of NaOH.