Physiology of Finfish and Shellfish (2+1)

Reagents

1. EDTA (Ethylene di-amine tetra acetate) – As anticoagulant
2. Ethyl alcohol – As fixing agent.
3. Giemsa stock solution
• Giemsa = 0.8 g
• Glycerol = 50 ml
• Methanol = 50 ml
• Above three are well mixed and filtered
4. Phosphate buffer
• Na₂HPO₄ (0.2 m) = 2.8 g / 100 ml
• Citric acid (0.1M) = 2.1 g / 100 ml
• Mix 40.9 ml Na₂HPO₄ and 9.1 ml citric acid and make the volume to 100ml.
5. Working solution
• It is prepared by mixing Giemsa stock solution, phosphate buffer solution and methanol.
• Giemsa stock solution = 5ml
• Phosphate buffer solution = 50ml
• Methanol = 1.5ml