Biotechnology and Bioinformatics (1+1)

3.1.4. Commonly used media for fish cell culture

• Medium 199, Eagle’s Minimum Essential Medium (MEM) and Eagle’s basal medium (BME).
• Other synthetic media suitable for fish cell culture are CMRL 1066, Leibovitz L-15, McLoy’s 5a, NCTC 109, and Puck’s medium.
• Among all these media MEM is suggested best for fish cell and tissue culture .
• In some cases with certain marine fish cell lines such as the grunt fin line, GF, it may be necessary to increase the NaCl concentrations of standard media.