Biotechnology and Bioinformatics (1+1)

3.1.9.1. Flow chart for dilution cloning

 Flow chart for dilution cloning

Monolayer of cells

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Remove the medium and add trypsin

(After few minutes the cells are in suspension)

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Add medium to the cells

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Count cells

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Dilute cells to 10-100 cells/ml

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Seed cells in a multi-well dish

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Let the cells settle down again

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Grow up clones for characterization

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Select clone

Here chemicals or monoclonal antibodies are used to kill other cells other than the desired cells.

Cell separation

This is an alternative way to cell cloning. Here cells are separated by means of their size, density, charge, surface area or specific affinities. For this flow cytometry, flow cytofluorimetry, fluorescence activated cell sorter (FACS) are used.