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3.1.7. Flow chart for primary cell culture from fin fish
3.1.7. Flow chart for primary cell culture from fin fish
Fish
Swab with 70% alcohol or betadine to sterilize the external surfaces
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Remove caudal fin, gills and scales aseptically
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Cut the tissues in to rate fine pieces aseptically
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Wash the tissues with phosphate bufferd saline (PBS) 2-3 times
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Place the washed tissue in a sterile
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Incubate at 28oC – 29oC for 24-72 hrs. The cells from the explant migrate into the surrounding medium and form a confluent monolayer
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A confluent monolayer may be formed in 3-4 days
Last modified: Thursday, 28 June 2012, 9:01 AM