Precipitation by organic solvents

PRECIPITATION BY ORGANIC SOLVENTS

  • To 1 ml of the protein solution, add 95% alcohol drop by drop.
  • Precipitation occurs due to removal of water of salvation around the protein molecule, as well as due to decrease in the dielectric constant of water, thus favoring protein –protein interaction.
Last modified: Wednesday, 23 March 2011, 8:09 AM