VPB 112: General Veterinary Biochemistry (1+1)

ESTIMATION OF AMINO ACIDS - PROCEDURE

Part I

Titration of amino acid + HCHO Vs NaOH

• Pipette out 5 ml of amino acid solution (glycine) into a 100 ml conical flask.
• Add 5 ml of formaldehyde solution and 2-3 drops of phenolphthalein as indicator.
• Titrate against the standard 0.02N NaOH solution till a permanent pale pink color appears.
• Note down the volume of NaOH utilized as (A).
• Repeat the experiment for concordant value.

Part II

Titration of HCHO Vs NaOH - Blank titration

• Pipette out 5 ml of the same formaldehyde solution into another 100 ml conical flask.
• Add 5 ml of distilled water and 2-3 drops of phenolphthalein as indicator.
• Titrate against the standard 0.02N NaOH solution till a permanent pale pink color appears.
• Note down the volume of NaOH utilized as (B).
• Repeat the experiment for concordant value.