VPB 112: General Veterinary Biochemistry (1+1)

ESTIMATION OF AMINO ACIDS - CALCULATION AND RESULT

Calculation

• Volume of NaOH consumed by amino acid V₁ = (A) – (B)
• Normality of NaOH N₁ = 0.02 N
• Volume of amino acid taken V₂ = 5.0 ml
• Normality of amino acid N₂ = ? (Use the formula V₁N₁=V₂N₂)
• The concentration of amino acid in the given solution = N₂ x Eq.Wt of the amino acid = N₂ X 75 (Eq.Wt of Glycine) = _________ g/L.

Result

• The concentration of Glycine in the given solution is _____________ g / L