VPB 121: Veterinary Physiology - II (2+1)

TOTAL PROTOZOAL COUNT - CALCULATION

Volume of 1 WBC large squares	= 0.1 cu mm
0.1 cu mm of SRL contains	= X protozoa
1 ml (1000 ml ) contains	= (X x 1000)/ 0.1 = X x 10,000 protozoa
Dilution rate	= 5 (5 ml of SRL is diluted to 25 ml)
So 1 ml of diluted SRL	= 1/5 = 0.2 ml of undiluted SRL
0.2 ml of undiluted SRL contains	= X x 10,000 Protozoa
1 ml of undiluted SRL contains	= X x 10,000 x (1/0.2) = X x 10,000 x 5 protozoa = X x 5 x 104

Result

• Number of protozoa per ml of SRL = x 10⁴