AGB 121: Principles of Animal Genetics and Population Genetics (2+1)

CHROMOSOME BANDING

• The chromosomes need to be stained in order to see them under a microscope.
• Several chromosome-banding techniques are used to enable the chromosome segments to be identify the chromosome.
  • Example: Quinacrine banding (Q-banding), Giemsa banding (G-banding), Reverse banding (R-banding), C-banding and Nucleolar Organizing Region stains (NOR stains).
• Most methods can distinguish between 300 and 450 bands in condensed chromosome.
• High resolution methods can distinguish up to 800 bands that can allow identification of small interstitial deletions.