VMC 221: Veterinary Immunology and Serology (1+1)

SINGLE DIFFUSION IN TWO DIMENSION
(MANCINI TEST)

• Antibody is incorporated into agar, poured into a glass plate to form a uniform layer.
• Circular wells are cut into the agar and antigen is introduced into the wells.
• Ring shaped bands of precipitates from concentrically around the well.
• This test is often used to quantitate the amount of antigen present and is read by measuring the size/area of the precipitin ring (figure).