VMC 221: Veterinary Immunology and Serology (1+1)

COUNTER IMMUNO ELECTROPHORESIS (CIE)/COUNTER CURRENT ELECTROPHORESIS/CROSS OVER ELECTROPHORESIS/COUNTER ELECTROPHORESIS

• Performed in agar gels.
• Gammaglobulins are exceptional in their cathodic migration. Most of the other proteins move to anode.
• By applying a voltage across the gel the antigen and antibody move towards each other and precipitate.
• The principle is same as in double diffusion, but is much faster and sensitivity is more.
• It can be used for the rapid diagnosis of many diseases.