VMC 221: Veterinary Immunology and Serology (1+1)

TWO DIMENSIONAL IMMUNOELECTROPHORESIS/TWO WAY IMMUNOELECTROPHORESIS

• In this method a strip of agarose containing electrophoretically resolved proteins is placed along one edge of the plate.
• Molten agarose containing antiserum is poured over the rest of the plate.
• When the plate has gelled, the antigens are forced into the antibody layer by electrophoresis.
• Migration is continued until each antigen is precipitated by its specific antibody .