Materials

MATERIALS

  • Antigen
  • Antisera
  • Glass slides or Petridish.
  • 1% agarose in normal saline. In case of poultry pathogens the composition is Agarose 1g; sodium chloride 8g, Distilled water – 100ml (To avoid contamination during storage and incubation add sodium azide 0.02g or merthiolate 1 in 10,000 concentration).
  • Template
  • Gel cutter
  • Needle
  • 50 µl micropipette & pipette tips
  • Normal saline
  • Staining solution.
    • Coomassie brilliant blue (R 250) 0.5 g.
    • Ethanol (96%) 45.0 ml
    • Glacial acetic acid 10.0 ml
    • Distilled water 45.0 ml
  • Destaining solution
    • Staining solution without Coomassie brilliant blue.
Last modified: Saturday, 24 September 2011, 4:46 AM