Materials

MATERIALS

  • Positive antigen, negative antigen, suspected antigen.
  • Hyper immune serum.
  • Barbitone buffer (Veronal buffer) pH 8.6
    • Tris  -  17.7g.
    • Barbitone sodium - 9.9g.
    • Sodium azide - 0.3g.
    • Distilled water - 2000 ml
    • pH adjusted 8.6 with concentrated HCI
  • Agarose slides – 0.8 to 1% agarose in barbitone buffer
  • Electrophoretic power pack and tank
  • Template
  • Gel cutter
  • Needle
  • Micropipettes and tips.
  • Filter paper strips.
  • 10% glacial acetic acid in water (v/v)
Last modified: Saturday, 24 September 2011, 4:52 AM