Procedure

PROCEDURE

  • Cut a well pattern as given in the template. The distance between the wells should be 4mm. Remove agarose from the well using the needle and place them on the electrophoresis tank.
  • Fill the buffer tanks with barbitone or Manthoney's  buffer.
  • Link each end of the slide to the electric circuit by filter paper strips pre soaked in barbitone or Manthoney's   buffer.
  • Wells towards cathode are filled with antigens (known +ve, known –ve and test antigen) and towards anode are filled with antiserum.
  • Electrophoresis is carried out using a current of 8 milliamperes / slide for 45-60 minutes.
  • Switch off and examine for white lines of precipitation between the pairs of wells. Readings are facilitated by oblique light.
  • Fix the protein by immersing the slide in 10% glacial acetic acid.
  • Stain the slide and observe for the presence of precipitation line. 

CIE Test

CIE Test

1= Antiserum
2 = Positive control antigen
3 = Test antigen (positive)
4= Negative control antigen
 
Last modified: Tuesday, 17 April 2012, 11:09 AM