Cultivation

CULTIVATION
  • They require living systems for multiplication.
  • They do not have cytochromes and their metabolic reactions are essentially anaerobic.
  • They are highly dependant on the host cells for energy.
  • Mouse inoculation
    • Suspected material is inoculated into mice intranasally, intra peritonelly.
    • The mice die within in ten days and smears of the lung, peritoneal exudates, spleen or brain will show the elementary bodies. (Levinthal cole Lillie or LCL bodies).
  • Inoculation on 6-7 days old embryonated eggs via the yolk sac route.
    • The LCL bodies can be demonstrated in the smears of yolk sac from infected eggs.
  • A variety of continuous cell lines are useful for cultivation of Chlamydiae, such as McCoy, BHk-21, L929 and vero.
    • Chemical treatment of the cells with cycloheximide (1-2mg/ml) and 5-iodo-2-deoxy uridine (80mg/ml) will greately enhance the cultivation.
Last modified: Monday, 4 June 2012, 5:52 AM