VPB 321: Animal Biotechnology (2+1)

IN VITRO FERTILIZATION IN FARM ANIMALS 

• The in vitro matured oocytes are mixed with the capacitated sperms in a petridish and incubated at 37^ºC with 5 % CO₂.
• After 24 hours incubation, the cumulus mass is removed manually and examined for fertilzation indicated by the presence of 2 pronuclei. Further the fertilized oocytes were incubated for division.
• The morula stage embryos (16 cell stage) are transferred to recipient animals for further development of embryos.
• Among farm animals, invitro fertilization has been achieved in cattle, pig and goat; but the number of new born animals were limited.