VPB 321: Animal Biotechnology (2+1)

Advantages and disadvantages of microinjection

ADVANTAGES AND DISADVANTAGES OF MICROINJETION

Advantages 

• The amount of DNA delivered per cell is not limited by the technique and can be
  optimized. This improves the chance for integrative transformation
• The delivery is precise, again increasing the chance of integrative transformation.
• The small structures can be injected containing only a few cells and with high regeneration potential.
• Since it is a direct physical approach, it is host-range­ independent.

Disadvantages 

• Injection can cause damage that affects embryonic survival and can result in quite high mortalities.
• Only one cell is targeted per injection