VPT 321: Veterinary Neuropharmacology (2+1) copy 1

PROCEDURE

• Mice are used as the animal for experiment, weighed and sex noted and then placed on the hot plate. 
• The end point of the reaction time is either the animal try to jump out from the hot plate or lick the paws. 
• The time at which the animal is placed on the hot plate is recorded and the time at which the animal tries to lick the paws or try to jump out is noted for the calculation of the reaction time.
• The animals are then injected with the analgesic drug and the same procedure is followed and the time is recorded and the reaction time is calculated.  
• If the reaction time is increased by atleast 7 seconds from the normal reaction time it is considered positive for the analgesic effect.