Blood Protozoa in Leishman-Giemsa stained smears
|
BLOOD PROTOZOA IN LEISHMAN-GIEMSA STANIED SMEARS
|
Protozoa
|
Specimen used for smear
|
Appearance in stained smear
|
Babesia canis
|
|
Blue, pear-shaped organisms, singly or in pair in erythrocytes
|
Hepatozoon canis
|
|
Rectangular blue-staining organisms (3 x 6 µm) with reddish nuclei in neutrophils - gelatin capsule like organism
|
Trypanosoma spp
|
Buffy coat (thick smears), lymph node aspirate or ascitic fluid
|
Long, flattened, spindle shaped trypomastigote (15-20 m m in length) forms, with central nucleus, an undulating membrane and a free flagellum, free in the blood, extracellular
|
Theileria annulata
|
|
Small rings, rods and commas many or single found in RBCs organisms stain bluish purple
|
Theileria spp
|
Lymph node aspirate smear
|
Schizonts (KBB) appear as roundish multineucleate structure in the cytoplasm of lymphoid cells organisms stain bluish purple
|
Ehrlichia spp
|
|
Morula stage (purple colour) in monocytes and lymphocytes
|
Anaplasma sp
|
|
Bright dots with a tendency to be located centrally or marginally. They may have a faint halo around them.
|
- 1-10 parasites/100 field = + (mild)
- 11-100 parasites/100 field = + + (moderate)
- 1-10 parasites/field = + + + (severe)
- >10 parasites/field = + + + + (very severe)
|
Last modified: Monday, 4 June 2012, 5:38 AM