VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

HANDLING DURING AND AFTER THAWING OF FROZEN SEMEN

• The semen straw should be taken with pre-cooled forceps.
• The straw should be given a jerk to remove all the liquid nitrogen attached over the surface.

Thaw the semen straw at 37 ⁰ C water bath either horizontally or vertically for 30 seconds.

• Don’t thaw more than 2-3 straws at a time.
• After thawing the semen should be used immediately.
• The straw should be wiped thoroughly to remove all the water.
• The semen straw should be cut at laboratory seal end where the air space is available.
• The AI gun should be loaded correctly after pulling the plunger down.
• The sheath should be applied over the AI gun and the button should be placed on the sheath.
• The semen should be deposited by pushing the plunger smoothly
• Do's and Don't in handling of liquid nitrogen

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