VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

GLASS WARES

Glass wares - sterilized in hot air oven at 160^oC for 1 hour or 180^oC for 30 minutes.

• The glass wares (conical flask, measuring cylinder, beaker etc.) has to be immeresed in neutral detergent solution immediately after their use.
• They should be washed washed throughly in running tap water and soaked in warm, non-spermicidal detregent solution for about 30 minutes.
• Subsequently these are thoroughly brushed with appropriate nylon brush, wahed in running tap water and finally again three times with double distilled water.
• The washed glass wares were allowed to dry by keeping them on inverted position on a blotting paper.
• Dried glass wares were covered with aluminium foil and sterilized in hot air oven at 160°C for 1 h.