VGO 511: Veterinary Andrology and Reproductive Techniques (1+1)

DILUENT

 

• Diluent is prepared on the day of semen collection.
• Prepare under laminar air flow unit in a hygienic manner.

• The dilutor should be prepared fresh on the day of semen collection early in the morning.
• Sometimes it can be prepared in the previous day evening but it should be noted that the antibiotics should be added only in the morning prior to use.
• Diluent should be prepared under laminar air flow unit (LAFU).
• Egg purchase, storage and the egg yolk preparation should be done as per standard procedure.
• After adding the egg yolk to the buffer, it should be stirred for 30 minutes for complete mixing of egg yolk to the buffer.