VLD 421: Veterinary Clinical Biochemistry and Laboratory Diagnosis-II (0+1)

PRINCIPLE

• In Dot Immunoassay nitrocellulose membrane is used as an adsorbent matrix instead of plastic surface.
• The antigen is dotted onto the nitrocellulose membrane and the membrane then incubated with test antibody, and secondly with enzyme conjugated antibody directed against the first antibody.
• In case of positve reaction, (specific antigen –antibody reaction) color  dot is developed against the white background at the site of antigen deposition.
• In case of negative reaction, no color development takes place at the site of antigen deposition .