Methods
Spot Test
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This test is used for rapid detection of the presence of a haemaggglutinating virus. Mixing two or three drops of suspected sample with equal quantity of 5% chicken red blood cells in normal saline. The appearance of the clumps of agglutinated red cells, indicate the presence of a haemagglutinating virus in the sample.
Micro Haemagglutination Test
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Dispense 25 µl of Phosphate buffer saline / normal saline into (one row for each sample) twelve wells of the plate.
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Add 25 µl of sample into the first well.
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Use a multichannel pipette to make two-fold serial dilutions along the row until the second last well from the end.
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The last well is the RBC control. Do not dilute this well.
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Gently tap the sides of the microwell plates to mix the reagents.
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Add 25 µl of 1 percent washed red blood cells to each well including the control wells in the last column.
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Gently tap the sides of the microwell plates to mix the reagents. Cover the plates with a lid. Allow to stand at room temperature for 30 -40 minutes.
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Last modified: Tuesday, 23 August 2011, 11:28 AM