VLD 421: Veterinary Clinical Biochemistry and Laboratory Diagnosis-II (0+1)

Qualitative test for nitrite in the blood

QUALITATIVE TEST FOR NITRITE IN BLOOD

Aim

• To detect the presence of nitrite in the blood.

Procedure

• Draw blood (10 ml) without anticoagulant from an affected animal and also from a known normal animal in 20 ml capacity test tubes.
• Place them in boiling water bath for 45 minutes. Cool them
• Observe the colour of the blood and the surface

Inference

• Blood sample containing nitrite is Salmon pink in colour, does not pull away from the side and the surface is level or concave.
• Normal blood sample is chocolate brown , pulls away from the side of tube and the surface is convex.