VMC 211: General Veterinary Microbiology (1+1)

EMBRYONATED EGGS

• One of the most economical and convenient methods  for growing many animal viruses is the chicken embryo inoculation. This method was devised in 1931.
• Different routes of inoculation are used namely yolk sac, allantoic, amniotic, chorioallantoic and intravenous route. Chick embryos have different types of cells in which various viruses can replicate. This method has been used in the production of vaccines against various animal and   human diseases.
• Limitation is that not all animal viruses can be  grown by this method. In the case of poultry viruses presence maternal antibody in embryonated chicken eggs may interfere with virus multiplication.