VMC 211: General Veterinary Microbiology (1+1)

CYTOPATHIC EFFECTS(CPE)

• Virus growth in cell culture can be detected by CPE. The CPE produced by some viruses are cell death, formation of giant cells, inclusion bodies etc.
• Inclusion bodies are aggregates of unassembled virus subunits or intact virus.  They may be intracytoplamic or intranuclear and may be acidophilic or basophilic.  Eg: Negri bodies in rabies - intracytoplasmic acidophilic inclusion bodies.
• Plaque: Infected area surrounded by normal cells in  monolayer cell cultures.
• Pock:  Infected area surrounded by normal area in chorioallantoic membrane.