VMC 211: General Veterinary Microbiology (1+1)

PRIMARY CULTURE

• When normal tissue is processed and grown in vitro the first monolayer formed is called a primary culture. Monolayer is a confluent layer of cell growing on the surface of a tissue culture flask. Cells from subcultures are called secondary cultures.
•  Primary cultures can subcultutred only a limited number of times before cell death. For some types of cells only a few divisions are possible  while in some 50-100 divisions can occur.Cultures from embryonic tissues are capable of greater number of divisions than those from adult tissues.