General Parasitology and Helminthology: Knott’s concentration method

Knott’s concentration method

KNOTTS CONCENTRATION METHOD

This is the standard test used to screen microfilariae in blood which includes lysis of erythrocytes, fixation and staining of larvae.

Procedure

Mix 1 ml of heparinized or EDTA treated blood with 9 ml of 2% formalin in a 15 ml tube, mix by inverting the tube several times after closing the tube with thumb.
Centrifuge the tube for 5 min at 1500 rpm, then discard the supernatant.
Add 1 or a few drops of 0.1% methylene blue or 0.2% methyl green solution to the sediment and mix well.
Remove a drop of stained sediment with a pipette, spread it on a slide and place a cover slip.
Examine all the sediment, drop by drop, under the microscope.

*** Note that heparinized (EDTA-treated) and formalinized blood samples are suitable for mailing to a laboratory for identification of microfilariae.

Last modified: Tuesday, 17 April 2012, 6:23 AM

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VPA 211: General Veterinary Parasitology and Helminthology (3+1)

Knott’s concentration method

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