General Parasitology and Helminthology: Haematocrit method

Haematocrit method

HAEMATOCRIT METHOD

Procedure

Fill a heparinized microhaematocrit tube with blood and centrifuge for 1 minute at 1500 rpm.
Place the tube under a dissecting microscope and examine the interface of the plasma and buffy coat for motility. Microfilariae (and haemoflagellates) will be observed actively swimming in the plasma.
Break the tube at the buffy coat interface and dab the buffy coat and a small amount of plasma on a slide; add a coverslip and examine for live microfilariae (at 10X) or allow it to air dry, fix for 1 min with methyl alcohol and stain with Giemsa for 30 min for identification (1.5 ml Giemsa’s stain in 15 ml distilled water buffered to pH 7.2).

*** Note that heparinized and non-fixed (non-formalin treated) blood specimens should be examined on the day of collection.

Last modified: Tuesday, 17 April 2012, 6:23 AM

Home
- Site pages
  - Tags
  - Calendar
  - Site news
- Current course
  - General Parasitology and Helminthology
    - Participants
    - General
    - Topic 1
    - Topic 2
    - Topic 3
    - Topic 4
    - Topic 5
    - Topic 6
    - Topic 7
    - Topic 8
    - Topic 9
    - Topic 10
    - Topic 11
    - Topic 12
    - Topic 13
    - Topic 14
    - Topic 15
    - Topic 16
    - Topic 17
    - Topic 18
    - Topic 19
    - Topic 20
    - Topic 21
    - Topic 22
    - Topic 23
    - Topic 24
    - Topic 25
    - Topic 26
    - Topic 27
    - Topic 28
    - Topic 29
    - Topic 30
    - Topic 31
    - Topic 32
    - Topic 33
    - Topic 34
    - Topic 35
    - Topic 36
    - Topic 37
    - Topic 38
    - Topic 39
    - Topic 40
    - Topic 41
    - Topic 42
    - Topic 43
    - Topic 44
    - Topic 45
    - Topic 46
    - Topic 47
    - Topic 48
    - Topic 49
    - Topic 50
    - Topic 51
    - Topic 52
    - Topic 53
    - Topic 54
      - Examination of faeces
      - Macroscopic examination
      - Microscopic examination
      - Direct examination of faecal sample
      - Concentration methods
      - Sedimentation technique
      - Floatation technique
      - Interpretation of microscopic examination of faeca...
      - Examination of urine
      - Examination of blood for detection of microfilaria
      - Knott’s concentration method
      - Haematocrit method
      - Cellophane tape preparation
      - Examination of nasal washings
    - Topic 55
    - Topic 56
    - Topic 57
    - Topic 58
    - Topic 59
    - Topic 60
    - Topic 61
    - Topic 62
    - Topic 63
    - Topic 64
    - Topic 65
    - Topic 66
    - Topic 67
    - Topic 68
    - Topic 69
- Courses

VPA 211: General Veterinary Parasitology and Helminthology (3+1)

Haematocrit method

Navigation