VMC 221: Veterinary Immunology and Serology (1+1)

OTHER SKIN TESTS

• For diagnosis of brucellosis (Brucella abortus), brucellin- a filtrate of a 20-day old broth culture and brucellergen – a nucleoprotein extract are used but they produce antibody and can not be used where eradication is monitored by serological tests.
• Mallein (culture filtrate), used for diagnosis of Glanders caused by Burkholderia mallei in horses. Short thermal test or ophthalmic test can be done.
• Antigen dropped to eye show transient conjunctivitis in positive cases.
• In the intrapalpebral test, antigen injected into skin of lower eyelid and positive reactions are indicated by swelling and ophthalmia.
• Skin testing also used for detection of histoplasmosis (using histoplasmin), coccidioidomycosis (using coccidioidin) toxoplasmosis (using toxoplasmin) and leprosy (Lepromin or leprosin).