VMC 221: Veterinary Immunology and Serology (1+1)

‘O’ ANTIGEN

• A smooth colony of a Salmonella culture is selected and inoculated into 10ml broth and incubated at 37 ⁰ C for 6 hours.
• Three or four agar plates were inoculated with the 6 hour old culture and incubated at 37 ⁰ C for 18 hours.
• The bacterial growth was harvested with saline and mixed thoroughly.
• The bacterial suspension was heated for 30 min in flowing steam (100⁰C) .
• Formalin 0.5% is added to the antigens as preservative.
• The antigen may be stored in the refrigerator.