VMC 221: Veterinary Immunology and Serology (1+1)

PRINCIPLE

• The principle of the test is that antibodies possess two antigen binding sites that will attach specifically with the antigenic determinants on the surfaces of the bacteria, cell or particles.
• Under suitable conditions one antibody molecule will combine with the determinant group on the surfaces of two bacteria and in this way a crystal lattice is formed. These lattices are generally visible with the naked eye as clumps and they sediment readily due to the large size of the clump.