Plate Agglutination test

PLATE AGGLUTINATION TEST

  • This is employed to screen sera within a short period for preliminary testing. This test is commonly used for screening of antibody against Mycoplasma gallisepticum, Salmonella Pullorum , Salmonella Gallinarum and Brucella abortus.

Materials

  • Brucella abortus colored antigen
  • Suspected serum
  • Glass plate

Procedure

  • Place one drop of the suspected serum and one drop of Brucella abortus antigen adjacent to each other on a glass plate.
  • Mix the reagents with an inoculation loop and rotate the plate while tilting the sides slowly. Watch for the agglutination reaction
  • Check the antigen for auto agglutination by replacing the serum with a loopful of saline.

  • Known negative and positive control serum should used simultaneously.

Plate agglutination

Source: http://www.poultry-health.com/library/serodiss/agglut2.jpg

Interpretation

  • Agglutination occurs within 4 minutes in positive cases.
Last modified: Tuesday, 17 April 2012, 10:18 AM