VMC 221: Veterinary Immunology and Serology (1+1)

Abortus bang ring test (ABRT)

ABORTUS BANG RING TEST

• This test is also known as Milk Ring Test (MRT). This test is employed to test milk from brucella infected cow/buffalo herd.

Materials

• Milk from brucella infected and non-infected herd
• Tetrazolium stained B. abortus antigen 
• Test tubes for conducting the test

Procedure

• Pipette 2 ml of milk in 2 different tubes from each sample. Add 1 drop of brucella ring test antigen and mix thoroughly.
• Incubate the milk for 1 hour at room temp, or for 30 minutes at 37 ⁰ C in a water bath.
• Observe the result .

Interpretation

• The clumps of agglutinated organisms are carried to the surface of the milk along with fat globules.
• A positive test is indicated by a column of discolored milk with a red cream layer on the top.
• A negative test will show red colour of the milk throughout with an uncolored cream layer on tap.