Fibrin and its Products

FIBRIN AND ITS PRODUC

Fibrinogen

  • Blood collected with anticoagulant is centrifuged to obtain plasma.
  • This plasma is buffered with sodium citrate acetic acid buffer with simultaneous addition of aqueous ethanol until pH of the solution becomes 7.2 and ethanol concentrates reaches 8%.
  • Now fibrinogen is precipitated and removed by centrifugation.
  • It is filtered, sterilized, freeze-dried and stored.

Fibrin foam

  • Plasma is suitably treated with citrate buffer of pH 6.
  • The solution is clarified by centrifugation and diluted to contain less than 2% fibrinogen and pH is adjusted to 6.25.
  • The solution is vigorously beaten to obtain fibrinogen foam.
  • This is converted to fibrin by the addition of thrombin.
  • The fibrin foam is frozen, cut into cubes and then freeze-dried.
  • Fibrin foam is white to cream coloured spongy material.
  • It can soak up liquid upto 30 times its weight and is very useful in nerve as well as arterial surgery and prostate operations.
  • It does not inhibit the action of antibiotics and eventually digested in the body system.

Fibrin powder

  • It is used to arrest bleeding where coagulation is delayed in skin injuries.

Fibrin bioplasts

  • These are moulded materials resembling plastic, which are prepared from fibrin powder.
  • They can be moulded even as bone joints and need not be removed from the body.

Last modified: Thursday, 16 September 2010, 6:24 AM