Fibrin and its Products
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Fibrinogen
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Blood collected with anticoagulant is centrifuged to obtain plasma.
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This plasma is buffered with sodium citrate acetic acid buffer with simultaneous addition of aqueous ethanol until pH of the solution becomes 7.2 and ethanol concentrates reaches 8%.
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Now fibrinogen is precipitated and removed by centrifugation.
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It is filtered, sterilized, freeze-dried and stored.
Fibrin foam
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Plasma is suitably treated with citrate buffer of pH 6.
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The solution is clarified by centrifugation and diluted to contain less than 2% fibrinogen and pH is adjusted to 6.25.
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The solution is vigorously beaten to obtain fibrinogen foam.
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This is converted to fibrin by the addition of thrombin.
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The fibrin foam is frozen, cut into cubes and then freeze-dried.
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Fibrin foam is white to cream coloured spongy material.
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It can soak up liquid upto 30 times its weight and is very useful in nerve as well as arterial surgery and prostate operations.
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It does not inhibit the action of antibiotics and eventually digested in the body system.
Fibrin powder
Fibrin bioplasts
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These are moulded materials resembling plastic, which are prepared from fibrin powder.
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They can be moulded even as bone joints and need not be removed from the body.
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Last modified: Thursday, 16 September 2010, 6:24 AM