VLD 411: Veterinary Clinical Biochemistry and Laboratory Diagnosis - I (0+1)

UROBILINOGEN

• Urobilinogen is normally present in urine. But, it will not be present in the case of obstructive jaundice.

Ehrlich’s test

• Ehrlich’s aldehyde reagent
  • Paradimethlyaminobenzaldehyde – 2 g
  • 20 per cent aqueous solution of conc.HCl – 100 ml
    • Take 5 ml of the urine in a test tube
    • Add 0.5 ml of the Ehrlich’s aldehyde reagent
    • A pink colour is positive for urobilinogen.

Interpretation

• Decreased amount or absence of urine urobilinogen
  • Obstruction of biliary passages
  • Decreased destruction of erythrocytes
  • Impaired intestinal absorption
  • Nephritis
• Increased amount of urine urobilinogen
  • Hepatitis
  • Cirrhosis of liver
  • Haemolytic jaundice