Glucose
- Normal urine does not contain glucose. Although glucose passes through the glomerulus, it should be reabsorbed by the proximal convoluted tubules.
Benedict’s test
Principle
- Glucose is a reducing agent, which converts the cupric ions in the copper sulphate to cuprous ions, cuprous oxide.
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Take 5 ml of the reagent in a test tube and heat it (the original blue colour of the reagent should not be changed)
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Add 8 drops of urine to it and boil it carefully seeing that it does not spurt while boiling
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Development of greenish, greenish-yellow, yellow or red color is positive for sugar
![BQR-Glu-Test BQR-Glu-Test](http://14.139.56.154:82/file.php/182/Topic-11/BQR-Glu-Test1.JPG)
Interpretation - Glycosuria
- Emotional stress
- Diabetes mellitus
- Hyperthyroidism
- Acute and chronic pancreatic necrosis
- Hyperpituitarism
- Overactivity of adrenal cortex
- Shock
- Chronic liver disease
- Enterotoxaemia in sheep
- Brain tumors
- Rabies
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False positive reaction for glucose can occur if other reducing agents like antibiotics, lactose, ascorbic acid, salicylates, morphine, formaldehyde and uric acid are present.
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Last modified: Monday, 4 June 2012, 4:41 AM